(To see other currencies, click on price)
MORE ABOUT THIS BOOK
Main description:
This volume represents a valuable and readily reproducible collection of established and emerging techniques for neuronal cell death research. Conveniently divided into four parts, sections cover a series of techniques for the molecular, structural, functional and genomic characterization of dying neurons, a number of protocols that are of primary interest in neuropathology and in experimental neuropathology, a series of gene engineering techniques to obtain and manipulate neuronal stem cells and progenitors, to prepare HSV-1 vectors for the gene therapy, and to CNS transplantation of bone marrow stem cells, and finally, some very interesting protocols for the study of cell death in non-mammalian models. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Neuronal Cell Death: Methods and Protocols seeks to serve a large audience of scientists that are currently active in the field or are willing to enter such an exciting and still expanding area of neurobiology.
Contents:
1. Neuronal Cell Death: An Overview of its Different Forms in Central and Peripheral Neurons
Laura Lossi, Claudia Castagna, and Adalberto Merighi
Part I: Molecular, Sturctural, Functional, and Genomic Changes in Dying Neurons2. Cell Volume Regulation Monitored with Combined Epifluorescence and Digital Holographic MicroscopyNicolas Pavillon and Pierre Marquet
3. Flow Cytometric Analysis of DNA Synthesis and Apoptosis in Central Nervous System Using Fresh Cell Nuclei
Noelia Lopez-Sanchez and Jose M. Frade
4. Nuclear Signs of Pre-Neurodegeneration
Fernando C. Baltanas, Jorge Valero, Jose Ramon Alonso, Maria Teresa Berciano, and Miguel Lafarga
5. Multi-Parametric O2 Imaging in Three-Dimensional Neural Cell Models with the Phosphorescent Probes
Ruslan I. Dmitriev and Dmitri B. Papkovsky
6. Calcium Imaging in Neuron Cell Death
Maria Calvo, Carlos Villalobos, and Lucia Nunez
7. Monitoring Mitochondrial Membranes Permeability in Live Neurons and Mitochondrial Swelling through Electron Microscopy Analysis
Macarena S. Arrazola and Nibaldo C. Inestrosa
8. Real-Time Visualization of Caspase-3 Activation by Fluorescence Resonance Energy Transfer (FRET)
Silvia Alasia, Carolina Cocito, Adalberto Merighi, and Laura Lossi
9. Design and Cloning of Short Hairpin RNAs (shRNAs) into a Lentiviral Silencing Vector to Study the Function of Selected Proteins in Neuronal Apoptosis
Nadia Canu
10. Genomic Analysis Using Affymetrix Standard Microarray GeneChips (169 Format) in Degenerate Murine Retina
Sook Hyun Chung, Weiyong Shen, and Mark Gillies
11. Genomic Analysis of Transcriptional Changes Underlying Neuronal Apoptosis
Sebastiano Cavallaro
12. High-Throughput Cell Death Assays
Matthew E. Pamenter and Gabriel G. Haddad
PartII: Cell Death in Neuropathology and Experimental Neuoropathology
13. Staining of Dead Neurons by the Golgi Method in Autopsy Material
Stavros J. Baloyannis
14. Image Analysis Algorithms for Immunohistochemical Assessment of Cell Death
Stan Krajewski, Jeffrey Wang, Tashmia Khan, Jonathan Liu, Chia-Hung Sze, and Maryla Krajewska
15. In Vitro Oxygen-Glucose Deprivation to Study Ischemic Cell Death
Carla I. Tasca, Tharine Dal-Cim, and Helena Cimarosti
16. Laser Microbeam Targeting of Single Nerve Axons in Cell Culture
Nicholas Hyun, Linda Z. Shi, and Michael W. Berns
17. Real-Time Imaging of Retinal Cell Apoptosis by Confocal Scanning Laser Ophthalmoscopy
Eduardo M. Normando, Mohammad H. Dehabadi, Li Guo, Lisa A. Turner, Gaia Pollorsi, and M. Francesca Cordeiro
18. Targeted Toxicants to Dopaminergic Neuronal Cell Death
Huajun Jin, Arthi Kanthasamy, Dilshan S. Harischandra, Vellareddy Anantharam, Ajay Rana, and Anumantha G. Kanthasamy
Part III: Neural Stem Cells, Progenitors, and Gene Therapy Strategies
19. Stem Cells, Neural Progenitors, and Engineered Stem Cells
Raj R. Rao and Shilpa Iyer
20. Herpes Simplex Virus Type 1 (HSV-1)-Derived Recombinant Vectors for Gene Transfer and Gene Therapy
Peggy Marconi, Cornel Fraefel, and Alberto L. Epstein
21. Herpes Simplex Virus Type 1 (HSV-1)-Derived Amplicon Vectors for Gene Transfer and Gene Therapy
Cornel Fraefel, Peggy Marconi, and Alberto L. Epstein
22. Bone Marrow Transplantation for Research and Regenerative Therapies in the Central Nervous System
David Diaz, Jose Ramon Alonso, and Eduardo Weruaga
Part IV: Neuronal Death in Non Mammalian Models
23. Detection of Activated Caspase-8 in Injured Spinal Axons by Using Fluorochrome-Labeled Inhibitors of Caspases (FLICA)
Anton Barreiro-Iglesias and Michael I. Shifman
24. Generation of Zebrafish Models by CRISPR/Cas9 Genome Editing
Alexander Hruscha and Bettina Schmid
25. In Vivo Assessment of Neuronal Cell Death in Drosophila
Pierre Dourlen
26. Drosophila Model for Studying Phagocytosis Following Neuronal Cell Death
Boris Shklyar, Flonia Levy-Adam, and Estee Kurant
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2014
Pages: 350
Weight: 652g
Availability: Available
Subcategories: Neuroscience
From the same series
