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Main description:
The last fifteen years have witnessed the birth and maturation of many original methods and the development of protocols specific to single molecule measurements and their analysis, including techniques involving optical imaging, electron microscopy, optical and magnetic trapping, and developments in atomic force microscopy. In Single Molecule Enzymology: Methods and Protocols, experts in the field provide procedures which enable the extraction of detailed information about enzyme work cycles, their static and kinetic properties, and information about their location and activity within cells. The detailed volume offers practical advice on many aspects of single molecule enzymology and includes strategic overviews of interconnected methods involved in sample preparation, single molecule measurements, and data analysis. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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Authoritative and up-to-date, Single Molecule Enzymology: Methods and Protocols is intended for use within the diverse community of molecular biologists, biochemists, and biophysicists studying enzymes in detail and can be used by researchers planning their first single molecule study or to aid more experienced researchers in further developing their existing studies.Feature:
Serves as an ideal guide for the diverse community of molecular biologists, biochemists, and biophysicists studying enzymes in detail
Features cutting-edge, lab ready protocols
Includes key tips and expert implementation advice to ensure successful results
Back cover:
The last fifteen years have witnessed the birth and maturation of many original methods and the development of protocols specific to single molecule measurements and their analysis, including techniques involving optical imaging, electron microscopy, optical and magnetic trapping, and developments in atomic force microscopy. In Single Molecule Enzymology: Methods and Protocols, experts in the field provide procedures which enable the extraction of detailed information about enzyme work cycles, their static and kinetic properties, and information about their location and activity within cells. The detailed volume offers practical advice on many aspects of single molecule enzymology and includes strategic overviews of interconnected methods involved in sample preparation, single molecule measurements, and data analysis. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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Authoritative and up-to-date, Single Molecule Enzymology: Methods and Protocols is intended for use within the diverse community of molecular biologists, biochemists, and biophysicists studying enzymes in detail and can be used by researchers planning their first single molecule study or to aid more experienced researchers in further developing their existing studies.Contents:
1. Single Enzyme Studies: A Historical Perspective
           Alex E. Knight
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2. Functional Surface-Attachment in a Sandwich Geometry of GFP-Labeled Motor Proteins
           Volker Bormuth, Felix Zörgibel, Erik Schäffer, and Jonathon Howard
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3. Studying Kinesin’s Enzymatic Cycle Using a Single-Motor Confocal Motility Assay, Employing Förster Resonance Energy Transfer
           Zdenek Lansky and Erwin J.G. Peterman
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4. Fluorescence Imaging with One Nanometer Accuracy: In Vitro and In Vivo Studies of Molecular Motors
           Melinda Tonks Hoffman, Janet Sheung, and Paul R. Selvin
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5. Snapshots of Kinesin Motors on Microtubule Tracks
           Franck J. Fourniol and Carolyn A. Moores
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6. Structural and Dynamic Characterization of Biochemical Processes by Atomic Force Microscopy
           Frédéric Eghiaian and Iwan A.T. Schaap
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7. Using Optical Tweezers to Study Fine Details of Myosin ATPase Mechanochemical Cycle
           Christopher Batters and Claudia Veigel
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8. Quantum Dot Labeling Strategies to Characterize Single Molecular Motors
           Shane R. Nelson, M. Yusuf Ali, and David M. Warshaw
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9. Imaging Individual Myosin Molecules Within Living Cells
           Tatiana A. Nenasheva, Gregory I. Mashanov, Michelle Peckham, and Justin E. Molloy
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10. Single Molecule Measurements Using Microneedles
           Toshio Yanagida, Yoshiharu Ishii, and Akihiko Ishijima
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11. Fluorescent Nucleoside Triphosphates for Single Molecule Enzymology
           Christopher P. Toseland and Martin R. Webb
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12. Probing the Mechanics of the Complete DNA Transcription Cycle in Real-Time Using Optical Tweezers
           Christoph G. Baumann and Stephen J. Cross
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13. A Single-Molecule Approach to Visualize the Unwinding Activity of DNA Helicases
           Natalia Fili, Christopher P. Toseland, Mark S. Dillingham, Martin R. Webb, and Justin E. Molloy
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14. Real-Time Single-Molecule Observation of Green Fluorescent Protein Synthesis by Immobilized Ribosomes
           Ryo Iizuka, Takashi Funatsu, and Sotaro Uemura
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15. Single-Molecule Measurements of Topoisomerase Activity with Magnetic Tweezers
           Yeonee Seol and Keir C. Neuman
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16. Assembly of Recombinant Nucleosomes on Nanofabricated DNA Curtains for Single Molecule Imaging
           Ja Yil Lee and Eric C. Greene
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17. Simultaneous Observation of Chemomechanical Coupling of a Molecular Motor
           Takayuki Nishizaka*, Yuu Hashimoto, and Tomoko Masaike
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18. Microsecond Resolution of Single Molecule Rotation Catalyzed by Molecular Motors
           Tassilo Hornung, James Martin, David Spetzler, Robert Ishmukhametov, and Wayne D. Frasch
PRODUCT DETAILS
Publisher: Springer (Humana Press)
Publication date: August, 2011
Pages: 300
Weight: 766g
Availability: Not available (reason unspecified)
Subcategories: Biochemistry
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