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Main description:
Central to the synthesis of proteins, the performance of catalysis, and many other physiological processes, the aberrant expression of which can be linked to human diseases including cancers, RNA has proven to be key target for therapeutics as well as a tool for therapy. In RNA Therapeutics: Function, Design, and Delivery, expert contributors from a broad spectrum of scientific backgrounds highlight the roles that messenger RNAs and small RNAs can play in biology and medicine. While covering the five major RNA-based drugs, namely the use of ribozymes to cleave and/or correct mRNA transcript, the use of siRNA for targeted silencing of gene transcripts, the use of aptamers, like short RNA molecules, for neutralizing the protein functions, the use mRNA-transfected DCs to activate immune system against tumor cells, as well as the use of RNA to reprogram T and/or DC cell function, this extensive volume brings together the fields of coding (mRNA) and non-coding RNA such as ribozymes, RNAse P, siRNAs, and miRNAs into one convenient source. Written in the highly successful Methods in Molecular Biology (TM) series format, the cutting-edge protocol chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and practical tips on troubleshooting and avoiding known pitfalls. Also, the book contains several excellent reviews for teaching purposes.
Authoritative and comprehensive, RNA Therapeutics: Function, Design, and Delivery provides key models and tools which will assist researchers in increasing our understanding of RNA functions, modifications, and their involvement in diseases in order to lead to the design of vital new RNA-based therapeutics.
Contents:
1. RNA Modifications: A Mechanism that Modulates Gene Expression
John Karijolich, Athena Kantartzis, and Yi-Tao Yu
2. Quantitative Analysis of RNA Modifications
John Karijolich, Athena Kantartzis, and Yi-Tao Yu
3. Advances in RNA Sensing by the Immune System: Separation of siRNA Unwanted Effects from RNA Interference
Mouldy Sioud
4. Progress in siRNA Delivery: A Multifunctional Approach by Polymeric Nanoparticles
Weiwei Gao, Zeyu Xiao, Alex Radovic-Moreno, Jinjun Shi, Robert Langer, and Omid C. Farokhzad
5. Optimized Protocols for siRNA Delivery into Monocytes and Dendritic Cells
Anne Mobergslien and Mouldy Sioud
6. Systemic Delivery of Synthetic siRNAs
Dag R. Sorensen and Mouldy Sioud
7. What Are the Key Targeted Delivery Technologies of siRNA Now?
Mouldy Sioud
8. Using OligoWalk to Identify Efficient siRNA Sequences
David H. Mathews
9. Jet-Injection of Short Hairpin RNA Encoding Vectors into Tumor Cells
Wolfgang Walther, Ulrike Stein, and Hermann Lage
10. Short Hairpin RNA (shRNA): Design, Delivery, and Assessment of Gene Knockdown
Chris B. Moore, Elizabeth H. Guthrie, Max Tze-Han Huang, and Debra Taxman
11. Effective Pol III-Expressed Long Hairpin RNAs Targeted to Multiple Unique Sites of HIV-1
Sheena M. Saayman, Patrick Arbuthnot, and Marc S. Weinberg
12. Functional Studies on RNA-Transfected Cell Microarrays
Christina Saeten Fjeldbo, Kristine Misund, Clara-Cecilie Gunther, Mette Langaas, Tonje Strommen Steigedal, Liv Thommesen, Astrid Laegreid, and Torunn Bruland
13. Transfection Microarrays for High-Throughput Phenotypic Screening of Genes Involved in Cell Migration
Reiko Onuki-Nagasaki, Akira Nagasaki, Kazumi Hakamada, Taro Q.P. Uyeda, Satoshi Fujita, Masato Miyake, and JunMiyake
14. Intron-Mediated RNA Interference, Intronic MicroRNAs, and Applications
Shao-Yao Ying, Chen Pu Chang, and Shi-Lung Lin
15. Inhibition of the microRNA Pathway in Zebrafish by siRNA
Anders Fjose and Xiao-Feng Zhao
16. Profiling of miRNA Expression and Prediction of Target Genes
Mouldy Sioud
17. Small RNA Cloning
Seungil Ro and Wei Yan
18. In situ Hybridization Detection of microRNAs
Rui Song, Seungil Ro, and Wei Yan
19. Detection and Quantitative Analysis of Small RNAs by PCR
Seungil Ro and Wei Yan
20. In Vivo Reprogramming of Human Telomerase Reverse Transcriptase (hTERT) by Trans-Splicing Ribozyme to Target Tumor Cells
Sang-Jin Lee, Seong-Wook Lee, Jin-Sook Jeong, and In-Hoo Kim
21. Design and Function of Triplex Hairpin Ribozymes
Guillermo Aquino-Jarquin, Ramiro Rojas-Hernandez, and Luis Marat Alvarez-Salas
22. Catalytic M1GS RNA as an Antiviral Agent in Animals
Yong Bai, Paul Jay Fannin Rider, and Fenyong Liu
23. Using Live Cells to Generate Aptamers for Cancer Study
Ling Meng, Kwame Sefah, Dalia Lopez Colon, Hui Chen, Meghan O'Donoghue, Xiangling Xiong, and Weihong Tan
24. Primer-Free Aptamer Selection Using a Random DNA Library
Weihua Pan and Gary A. Clawson
25. Development of TLR 7/8 Small RNA Antagonists
Mouldy Sioud
26. Modulation of Dendritic Cell Maturation and Function by siRNA-Bearing 5'-Triphosphate
Mouldy Sioud
27. Immunotherapy of Cancer with Dendritic Cells Loaded with Tumor Antigens and Activated through mRNA Electroporation
An M.T. Van Nuffel, Jurgen Corthals, Bart Neyns, Carlo Heirman, Kris Thielemans, and Aude Bonehill
28. Non-MHC-Dependent Redirected T Cells Against Tumor Cells
Hilde Almasbak, Marianne
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: September, 2010
Pages: 543
Weight: 2550g
Availability: Available
Subcategories: Biochemistry, Genetics
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