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Main description:
For more than a century, microscopy has been a centerpiece of extraordinary discoveries in biology. Along the way, remarkable imaging tools have been developed allowing scientists to dissect the complexity of cellular processes at the nano length molecular scales. Nanoimaging: Methods and Protocols presents a diverse collection of microscopy techniques and methodologies that provides guidance to successfully image cellular molecular complexes at nanometer spatial resolution. The book's four parts cover: (1) light microscopy techniques with a special emphasis on methods that go beyond the classic diffraction-limited imaging; (2) electron microscopy techniques for high-resolution imaging of molecules, cells and tissues, in both two and three dimensions; (3) scanning probe microscopy techniques for imaging and probing macromolecular complexes and membrane surface topography; and (4) complementary techniques on correlative microscopy, soft x-ray tomography and secondary ion mass spectrometry imaging. Written in the successful format of the Methods in Molecular Biology (TM) series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Nanoimaging: Methods and Protocols highlights many of the most exciting possibilities in microscopy for the investigation of biological structures at the nano length molecular scales.
Contents:
1. Introduction: Nanoimaging Techniques in Biology
Alioscka A. Sousa and Michael J. Kruhlak
Part I. Light Microscopy
2. Live-Cell Imaging of Vesicle Trafficking and Divalent Metal Ions by Total Internal Reflection Fluorescence (TIRF) Microscopy
Merewyn K. Loder, Takashi Tsuboi, and Guy A. Rutter
3. 4Pi Microscopy
Roman Schmidt, Johann Engelhardt, and Marion Lang
4. Fluorescence in situ Hybridization Applications for Super-Resolution 3D Structured Illumination Microscopy
Yolanda Markaki, Daniel Smeets, Marion Cremer, and Lothar Schermelleh
5. Two-Color STED Imaging of Synapses in Living Brain Slices
Jan Tonnesen, and U. Valentin Nagerl
6. Super-Resolution Imaging by Localization Microscopy
Dylan M. Owen, Astrid Magenau, David Williamson, and Katharina Gaus
7. High-Content Super-Resolution Imaging of Live Cell by uPAINT
Gregory Giannone, Eric Hosy, Jean-Baptiste Sibarita, Daniel Choquet, and Laurent Cognet
8. Super-Resolution Fluorescence Imaging with Blink Microscopy
Christian Steinhauer, Michelle S. Itano, and Philip Tinnefeld
9. Photoswitchable Fluorophores for Single-Molecule Localization Microscopy
Kieran Finan, Benjamin Flottmann, and Mike Heilemann
10. Single-Molecule Tracking of mRNA in Living Cells
Mai Yamagishi, Yoshitaka Shirasaki, and Takashi Funatsu
Part II. Electron Microscopy
11. Semiautomatic, High-Throughput, High-Resolution Protocol for Three-Dimensional Reconstruction of Single Particles in Electron Microscopy
Carlos Oscar Sorzano, J. M. de la Rosa Trevin, J. Oton, J. J. Vega, J. Cuenca, A. Zaldivar-Peraza, J. Gomez-Blanco, J. Vargas, A. Quintana, R. Marabini, and J. M. Carazo
12. Mass Mapping of Amyloid Fibrils in the Electron Microscope using STEM Imaging
Alioscka A. Sousa and Richard D. Leapman
13. Elemental Mapping by Electron Energy Loss Spectroscopy in Biology
Maria A. Aronova and Richard D. Leapman
14. Cellular Nanoimaging by Cryo Electron Tomography
Roman I. Koning and Abraham J. Koster
15. Large-Volume Reconstruction of Brain Tissue from High-Resolution Serial Section Images Acquired by SEM-Based Scanning Transmission Electron Microscopy
Masaaki Kuwajima, John M. Mendenhall, and Kristen M. Harris
16. 3D Imaging of Cells and Tissues by Focused Ion Beam/Scanning Electron Microscopy (FIB/SEM)
Damjana Drobne
17. Preparation of Gold Nanocluster Bioconjugates for Electron Microscopy
Christine L. Heinecke and Christopher J. Ackerson
Part III. Scanning Probe Microscopy
18. Atomic Force Microscopy Imaging of Macromolecular Complexes
S. Santos, D.J. Billingsley, and N.H. Thomson
19. Imaging of Transmembrane Proteins Directly Incorporated Within Supported Lipid Bilayers Using Atomic Force Microscopy
Daniel Levy and Pierre-Emmanuel Milhiet
20. Functional AFM Imaging of Cellular Membranes Using Functionalized Tips
Lilia A. Chtcheglova and Peter Hinterdorfer
21. Near-Field Scanning Optical Microscopy for High Resolution Membrane Studies
Heath A. Huckabay, Kevin P. Armendariz, William H. Newhart, Sarah M. Wildgen, and Robert C. Dunn
Part IV. Complementary Techniques
22. Correlative Fluorescence and EFTEM Imaging of the Organized Components of the Mammalian Nucleus
Michael J. Kruhlak
23. High Data Output Method for 3-D Correlative Light-Electron Microscopy Using Ultrathin Cryosections
Katia Cortese, Giuseppe Vicidomini, Maria C. Gagliani, Patrizia Boccacci, Alberto Diaspro, and Carlo Tacchetti
24. Correlative Optical and Scanning Probe Microscopies for Mapping Interactions at Membranes
Christopher M. Yip
25. Nanoimaging Cells Using Soft X-Ray Tomography
Dilworth Y. Parkinson, Lindsay R. Epperly, Gerry McDermott, Mark A. Le Gros, Rosanne M. Boudreau, and Carolyn A. Larabell
26. Secondary Ion Mass Spectrometry Imaging of Biological Membranes at High Spatial Resolution
Haley A. Klitzing, Peter K. Weber, and Mary L. Kraft
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: October, 2012
Pages: 526
Weight: 652g
Availability: Available
Subcategories: Radiology
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