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MORE ABOUT THIS BOOK
Main description:
Homing Endonucleases: Methods and Protocols aims at providing molecular biologists with a comprehensive resource to identify and characterize homing endonucleases from genomic sequence, to deduce the biological basis of binding and cleavage specificity, as well as to provide protocols to redesign endonuclease target specificity for genome-editing applications. Engineering of designer homing endonucleases has set the stage for genome editing of complex eukaryotic genomes with a broad range of potential applications including targeted gene knockouts in model organisms and gene therapy in humans, making this book a valuable resource for future research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Homing Endonucleases: Methods and Protocols serves as a key reference for all labs studying site-specific DNA endonucleases.
Feature:
Serves as a comprehensive resource to identify and characterize homing endonucleases and much more
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Back cover:
Homing Endonucleases: Methods and Protocols aims at providing molecular biologists with a comprehensive resource to identify and characterize homing endonucleases from genomic sequence, to deduce the biological basis of binding and cleavage specificity, as well as to provide protocols to redesign endonuclease target specificity for genome-editing applications. Engineering of designer homing endonucleases has set the stage for genome editing of complex eukaryotic genomes with a broad range of potential applications including targeted gene knockouts in model organisms and gene therapy in humans, making this book a valuable resource for future research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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Authoritative and practical, Homing Endonucleases: Methods and Protocols serves as a key reference for all labs studying site-specific DNA endonucleases.Contents:
1. Homing Endonucleases:Â From Genetic Anomalies to Programmable Genomic Clippers
           Marlene Belfort and Richard P. Bonocora
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2. Bioinformatic Identification of Homing Endonucleases and Their Target Sites
           Eyal Privman
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3. PCR-Based Bioprospecting for Homing Endonucleases in Fungal Mitochondrial rRNA Genes
           Mohamed Hafez, Tuhin Kumar Guha, Chen Shen, Jyothi Sethuraman, and Georg Hausner
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4. Mapping Homing Endonuclease Cleavage Sites Using In Vitro Generated Protein
           Richard P. Bonocora and Marlene Belfort
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5. Mapping Free-Standing Homing Endonuclease Promoters Using 5’RLM-RACE
           Ewan A. Gibb
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6. PCR Analysis of Chloroplast Double-Strand Break (DSB) Repair Products Induced by I-CreII in Chlamydomonas and Arabidopsis
           Taegun Kwon, Obed W. Odom, Weihua Qiu, and David L. Herrin
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7. A Two-Plasmid Bacterial Selection System for Characterization and Engineering of Homing Endonucleases
           Ning Sun and Huimin Zhao
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8. Rapid Screening of Endonuclease Target Site Preference Using a Modified Bacterial Two-Plasmid Selection
           Jason M. Wolfs, Benjamin P. Kleinstiver, and David R. Edgell
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9. A Yeast-Based Recombination Assay for Homing Endonuclease Activity
           Jean-Charles Epinat
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10. Rapid Determination of Homing Endonuclease DNA Binding Specificity Profile
           Lei Zhao and Barry L. Stoddard
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11. Quantifying the Information Content of Homing Endonuclease Target Sites by Single Base Pair Profiling
           Joshua I. Friedman, Hui Li, and Raymond J. Monnat, Jr.
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12. Homing Endonuclease Target Site Specificity Defined by Sequential Enrichment and Next-Generation Sequencing of Highly Complex Target Site Libraries
           Hui Li and Raymond J. Monnat, Jr.
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13. Homing Endonuclease Target Determination Using SELEX Adapted for Yeast Surface Display
           Kyle Jacoby and Andrew M. Scharenberg
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14. Engineering and Flow-Cytometric Analysis of Chimeric LAGLIDADG Homing Endonucleases from Homologous I-OnuI-Family Enzymes
           Sarah K. Baxter, Andrew M. Scharenberg, and Abigail R. Lambert
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15. Bioinformatics Identification of Coevolving Residues
           Russell J. Dickson and Gregory B. Gloor
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16. Identification and Analysis of Genomic Homing Endonuclease Target Sites
           Stefan Pellenz and Raymond J. Monnat, Jr.
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17. Redesigning the Specificity of Protein-DNA Interactions with Rosetta
           Summer Thyme and David Baker
PRODUCT DETAILS
Publisher: Springer (Humana Press)
Publication date: February, 2014
Pages: 300
Weight: 788g
Availability: Not available (reason unspecified)
Subcategories: Genetics
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