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Main description:
Glycoconjugates such as glycoproteins and glycolipids play important roles in cell-cell interaction events, including development, differentiation, m- phogenesis, fertilization, inflammation, and metastasis. A number of reports have documented the association of unique oligosaccharide sequences to p- tein targeting and folding, and in mechanisms of infection, inflammation, and immunity. For glycoproteins, these glycan appendages are the result of extensive co- or post-translational modifications of the nascent proteins in the endoplasmic reticulum and in the Golgi apparatus. Although nucleic acids and proteins are copied from a template in a repeated series of identical steps using the same enzymes, complex carbohydrates are formed by the sequential actions of cellular glycosyltransferases that specifically recognize unique s- strates. The molecular biology of these transferases and other carbohydra- modifying enzymes is providing important insights on oligosaccharide recognition events.
While it is acknowledged that the definition of the protein complement of cells and tissues (the so-called proteome) remains an enormous task in this postgenomic era, the characterization of all glycans produced by individual organisms (referred to as the glycome) presents an equally imp- tant challenge. This task is further complicated by the fact that oligosacc- rides cannot presently be cloned. These complex carbohydrates exist in a staggering diversity of structures, linkages, and branching, thus providing an exquisite molecular repertoire for cellular interactions.
Contents:
Part I. Introduction
Structural and Functional Diversity of Glycoconjugates: A Formidable Challenge to the Glycoanalyst
Gerald W. Hart
Part II. Sample Preparation Methods
Chemical and Enzymatic Release of Glycans from Glycoproteins
Tony Merry and Sviatlana Astrautsova
Derivatization of Carbohydrates
Shigeo Suzuki, John F. Kelly, Steven J. Locke, Pierre Thibault, and Susumu Honda
Preparation of Oligosaccharides from Sulfated Glycosaminoglycans Using Bacterial Enzymes
Shuhei Yamada and Kazuyuki Sugahara
Part III. Analysis of Mono- and Oligosaccharides
Monosaccharide Analysis by Capillary Electrophoresis
Susumu Honda
Separation of Oligosaccharides by Capillary Electrophoresis Using Buffer Modifiers
Susumu Honda and Shigeo Suzuki
Characterization of Oligosaccharides from Starch, Dextran, Cellulose, and Glycoproteins by Capillary Electrophoresis
Fu-Tai A. Chen
Affinity Capillary Electrophoresis of Oligosaccharides
Atsushi Taga and Susumu Honda
Characterization of Glycosaminoglycans by Capillary Electrophoresis
Robert J. Linhardt and Toshihiko Toida
Part IV. Analysis of Glycoconjugates and the Component Monosaccharides
Analysis of 2-Aminoacridone-Derivatized Complex Oligosaccharides Using Micellar Electrokinetic Capillary Chromatography and Laser-Induced Fluorescence Detection
Helen C. Birrell and Patrick Camilleri
Separation of Protein Glycoforms by Capillary Electrophoresis
Myriam Taverna, Nguyet Thuy Tran, and Danielle Ferrier
Separation of Glycoproteins by Capillary Isoelectric Focusing
Ira S. Krull, Sarah Kazmi, Huijuan Zhong, and Ling Chen Santora
Glycoprotein Analysis by Capillary Electrophoresis-Electrospray Mass Spectrometry
Kevin P. Bateman, John F. Kelly, Pierre Thibault, Louis Ramaley, and Robert L. White
Analysis of Bacterial Glycolipids by Capillary Electrophoresis-Electrospray Mass Spectrometry: Haemophilus influenzae and Neisseria meningitidis Lipopolysaccharides
Pierre Thibault, Adele Martin, Michel Gilbert, Warren W. Wakarchuk, and James C. Richards
Part V. Specific Applications
Capillary Electrophoresis as an Assay Method for Monitoring Glycosyltransferase Activity
Warren W. Wakarchuk and Anna-Maria Cunnningham
Determination of Association Constant of Carbohydrate-Protein Interaction
Atsushi Taga and Susumu Honda
Appendix
Structures of Carbohydrates Found in Animals and Bacteria
Shigeo Suzuki, Malcolm Perry, Pierre Thibault, and Susumu Honda
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 334
Weight: 510g
Availability: Available
Subcategories: Biochemistry
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