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Main description:
Featuring current resources used to discover new legume family genes and to understand genes and their interactions, Legume Genomics: Methods and Protocols provides techniques from expert researchers to study these plants that are so vitally important for food, feed, human nutrition, bioenergy, and industrial purposes. This detailed volume covers genome characterization and analysis, transcriptome analysis and miRNA identification/analysis, forward and reverse genetics, molecular markers, as well as transformation strategies used to investigate gene function and many other topics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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Authoritative and useful, Legume Genomics: Methods and Protocols aims to serve plant molecular biologists, molecular breeders, plant physiologists and biochemists, developmental biologists, and those interested in plant-microbe interactions.Feature:
Features cutting-edge methods and protocols examining genes and gene interactions in legumesÂ
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Back cover:
Featuring current resources used to discover new legume family genes and to understand genes and their interactions, Legume Genomics: Methods and Protocols provides techniques from expert researchers to study these plants that are so vitally important for food, feed, human nutrition, bioenergy, and industrial purposes. This detailed volume covers genome characterization and analysis, transcriptome analysis and miRNA identification/analysis, forward and reverse genetics, molecular markers, as well as transformation strategies used to investigate gene function and many other topics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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Authoritative and useful, Legume Genomics: Methods and Protocols aims to serve plant molecular biologists, molecular breeders, plant physiologists and biochemists, developmental biologists, and those interested in plant-microbe interactions.Contents:
1. The Model Legume Genomes
           Steven B. Cannon
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2. Fluorescent In Situ Hybridization (FISH) on Pachytene Chromosomes as a Tool for Genome Characterization
           René Geurts and Hans de Jong
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3. Targeted Mutagenesis for Functional Analysis of Gene Duplication in Legumes
           Shaun J. Curtin, Justin E. Anderson, Colby G. Starker, Nicholas J. Baltes, Dhananjay Mani, Daniel F. Voytas, and Robert M. Stupar
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4. RNA-Seq for Transcriptome Analysis in Non-Model Plants
           Rohini Garg and Mukesh Jain
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5. Functional Analysis of Legume Genome Arrays
           Nicolas Goffard and Georg Weiller
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6. Genome-Wide Identification of MicroRNAs in Medicago truncatula by High-Throughput Sequencing
           Tian-Zuo Wang and Wen-Hao Zhang
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7. Determining Abundance of MicroRNAs and Other Small RNAs in Legumes
           Cecilia Contreras-Cubas, Alejandra A. Covarrubias, and José Luis Reyes
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8. Forward Genetics Screening of Medicago truncatula Tnt1 Insertion Lines
           Juan Carlos Serrani Yarce, Hee-Kyung Lee, Million Tadege, Pascal Ratet, and Kirankumar S. Mysore
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9. Reverse Genetics in Medicago truncatula Using a TILLING Mutant Collection
           Maria Carelli, Ornella Calderini, Francesco Panara, Andrea Porceddu , Ilaria Losini, Pietro Piffanelli, Sergio Arcioni, and Carla Scotti
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10. High-Throughput and Targeted Genotyping of Lotus japonicus LORE1 Insertion Mutants
           Dorian Fabian Urbański, Anna Małolepszy, Jens Stougaard, and Stig Uggerhøj Andersen
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11. Isolation and Forward Genetic Analysis of Developmental Genes in Pea
           James L. Weller, Valérie F.G. Hecht, and Frances C. Sussmilch
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12. Gene Silencing in Medicago truncatula Roots Using RNAi
           Daniela S. Floss, Alexa M. Schmitz, Colby G. Starker, J. Stephen Gantt, and Maria J. Harrison
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13. Molecular Markers for Genetics and Plant Breeding: The MFLP Marker System and Its Application in Narrow-Leafed Lupin (Lupinus angustifolius)
           Islam Shahidul, Huaan Yang, and Guijun Yan
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14. Stable Transformation of Medicago truncatula cv. Jemalong for Gene Analysis Using Agrobacterium tumefaciens
           Youhong Song, Kim E. Nolan, and Ray J. Rose
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15. Transient Gene Expression in Medicago truncatula Leaves Via Agroinfiltration
           Kelsey Picard, Robyn Lee, Roger Hellens, and Richard C. Macknight
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16. Subcellular Localization of Transiently-Expressed Fluorescent Fusion Proteins
           David A. Collings
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17. Proteomics and the Analysis of Nodulation
           Nijat Imin
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18. Phosphoproteomic Analysis of Peptides
           Mark A. Baker
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19. Plant Metabolomics: From Experimental Design to Knowledge Extraction
           Amit Rai, Shivshankar Umashankar, and Sanjay Swarup
PRODUCT DETAILS
Publisher: Springer (Humana Press)
Publication date: September, 2013
Pages: 325
Weight: 812g
Availability: Not available (reason unspecified)
Subcategories: Genetics
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