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MORE ABOUT THIS BOOK
Main description:
Macromolecular Crystallography Protocols, now in two volumes, examines major developments that have occurred since publication of the acclaimed first edition nearly a decade ago. Volume 1, Preparation and Crystallization of Macromolecules and Volume 2, Structure Determination, explore recent advances that have accelerated the pace of structural determination and made crystallography accessible to a broader range of investigators.
Volume 1 is composed of detailed protocols for the preparation and optimization of crystals, including tips from the experts on the best methods for inducing proteins to adopt their crystalline form. Volume 2 complements the first volume by addressing laboratory techniques for crystal handling and structural characterization, as well as computational techniques for data collection, phasing, and refinement. The volume concludes with a detailed and insightful survey of available crystallographic software. These volumes will be an indispensable reference for obtaining macromolecular crystals and determining their three-dimensional structure.
Feature:
Examines major developments that have occurred since publication of the acclaimed first edition nearly a decade ago
Concludes with a detailed and insightful survey of available crystallographic software
An indispensable reference for obtaining macromolecular crystals and determining their three-dimensional structure
Contents:
TABLE OF CONTENTS VOLUME 1
HUMANA PRESS-CRYSTALLOGRAPHIC METHODS AND PROTOCOLS
Sylvie Doublié, Editor
CHAPTER TITLE AUTHOR(S) PAGE
1.
A Generic Method for the Production of Recombinant Proteins in Escherichia coli Using a Dual His6-MBP Affinity Tag
Joseph E. Tropea, Scott Cherry, Sreedevi Nallamsetty, Christophe Bignon, and David S.Waugh
1-29
2. Cloning, production, and purification of proteins for a medium scale structural genomics project Sophie Quevillon-Cheruel, Bruno Collinet, Lionel Trésaugues, Philippe Minard, Gilles Henckes, Robert Aufrère, Karine Blondeau, Cong-Zhao Zhou, Dominique Liger, Nabila Bettache, Anne Poupon, Ilham Aboulfath, Nicolas Leulliot, Joel Janin, and Herman van Tilbeurgh
1-26
3. Baculoviral expression of an integral membrane protein for structural studies
Dean R. Madden and Markus Safferling 1-33
4. Protein Engineering Sonia Longhi, François Ferron, and Marie-Pierre Egloff
1-48
5. Selenomethionyl protein expression Sylvie Doublié 1-21
6. How to Use Dynamic Light Scattering to Improve Your Chances of Growing Crystals of a Macromolecule
Gloria Borgstahl 1-34
7. Screening and optimization methods for non-automated crystallization laboratories
Terese Bergfors 1-32
8. Improving Marginal Crystals Charles W. Carter, Jr. and
Madeleine Riès-Kautt
1-34
9. Optimisation Techniques for Automation and High Throughput
Naomi E. Chayen 1-26
10. Three-dimensional crystallization of membrane proteins
James Féthière 1-40
11. Crystallization of Protein-DNA complexes
Thomas Hollis 1-20
12. Preparation and crystallization of RNA
Barbara Golden 1-24
13. Crystallisation of RNA-protein complexes Eiji Obayashi, Chris Oubridge, Daniel Pomeranz Krummel, and Kiyoshi Nagai
1-27
PRODUCT DETAILS
Publisher: Springer (Humana Press)
Publication date: February, 2007
Pages: 280
Weight: 1310g
Availability: Not available (reason unspecified)
Subcategories: Biochemistry
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