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Main description:
Electroporation gene therapy, or gene electrotransfer, has evolved greatly over the last few decades as a result of the remarkable progress in genetic sequencing, gene array analysis, gene cloning, gene expression detection, DNA manufacture and discovery and synthesis of siRNA. Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition provides in-depth knowledge on the delivery of naked DNA and small-interfering RNA (siRNA) to the targeted cells, tissues, and animals for prevention and treatment of disease. It builds on the success of the first edition and on the progress made in siRNA delivery and DNA vaccines for large animals as well as discovery of electroporation applications for the fragile tissues and for internal organs. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition aims to provide not only comprehensive coverage of the basic theory and practical application of electroporation siRNA therapy, gene therapy, and vaccine, but also elaborates on the most current views from the experts in this field, serving as an invaluable resource for investigators both in and outside of this field.
Contents:
Part I. Basic Concepts and Enhancement of DNA Transfer via Electroporation
1. Electroporation-Based Gene Therapy: Recent Evolution in the Mechanism Description and Technology Developments
Lluis M. Mir
2. Electropermeabilization of the Cell Membrane
Justin Teissie
3. The Impact of Non-Electrical Factors on Electrical Gene Transfer
Jiemiao Hu, Jeffry Cutrera, and Shulin Li
4. Electroporation Formulation for Cell Therapy
Jiemiao Hu and Shulin Li
5. Short-Fragment DNA-Mediated in Vivo DNA Electroporation Delivery
Jinliang Peng, Yonggang Zhao, and Yuhong Xu
6. Gold Nanoparticle-Enhanced Electroporation for Leukemia Cell Transfection
Shuyan Huang, Yingbo Zu, and Shengnian Wang
Part II. SiRNA Electrotransfer
7. SiRNA Delivery via Electropulsation: A Review of the Basic Processes
Muriel Golzio and Justin Teissie
8. Continuous Cell Electroporation for Efficient DNA and siRNA Delivery Based on Laminar Microfluidic Chips
Zewen Wei and Zhihong Li
9. Electroporation of siRNA into Mouse Bone Marrow-Derived Macrophages and Dendritic Cells
Isabel Popp, Valentin Schatz, Alexander T. Prechtel, Alexander Steinkasserer, Christian Bogdan, and Jonathan Jantsch
10. Direct Imaging of siRNA Electrotransfer at the Single Cell Level
Justin Teissie and Muriel Golzio
11. Electroporation-Mediated siRNA Delivery into Tumors
Yoshifumi Takei
12. Substrate-Mediated, High Efficiency siRNA Electroporation
Hiroyuki Fujimoto and Hiroo Iwata
13. Intradermal Electroporation of RNA
Maria L. Knudsen, Karl Ljungberg, Peter Liljestroem, and Daniel X. Johansson
Part III. Electroporation-Mediated Delivery to Poorly Accessible and Fragile Tissues
14. Delivery of DNA into the Central Nervous System via Electroporation
Jochen De Vry, Tim Vanmierlo, Pilar Martinez-Martinez, Mario Losen, Yasin Temel, Harry Steinbusch, Marc De Baets, and Jos Prickaerts
15. DNA Delivery in Adult Mouse Eyes: An Update with Corneal Outcomes
John M. Nickerson, Shannon E. Getz, Jana T. Sellers, Micah A. Chrenek, Penny Goodman, Christiana J. Bernal, and Jeffrey H. Boatright
16. Delivery of Plasmid DNA into Developing Rat Teeth by Electroporation
Shaomian Yao, Michael L. Beckley, and Dawen Liu
17. Electroporation-Mediated Gene Delivery to the Lungs
Jennifer L. Young, Michael S. Barravecchia, and David A. Dean
18. Electroporation-Mediated Delivery of Genes in Rodent Models of Lung Contusion
David Machado-Aranda and Krishnan Raghavendran
19. In Vivo Electroporation-Mediated Gene Delivery to the Beating Heart
Hendrik T. Tevaearai, Amiq Gazdhar, M. N. Giraud, and Martin Fluck
Part IV. Treatment of Cancer in Large Animals and Humans via Electroporation Therapy
20. Managing Local Swelling Following Intratumoral Electro-Chemo-Gene Therapy
Jeffry Cutrera, Glenn King, Pamela Jones, Elias Gumpel, Xueqing Xia, and Shulin Li
21. Gene Electrotransfer in Clinical Trials
Julie Gehl
22. Electrochemotherapy in Veterinary Oncology: From Rescue to First-line Therapy
Enrico P. Spugnini and Alfonso Baldi
23. Low Energy DC Current Ablation in a Mouse Tumor Model
Prejesh Philips and Robert C. G. Martin
Part V. Applications of Electroporation for DNA Vaccination
24. Evolution of Electroporated DNA Vaccines
Andrea Keane-Myers and Matthew Bell
25. Clinical Development of Intramuscular Electroporation: Providing a "Boost" for DNA Vaccines
Amir S. Khan, Kate E. Broderick, and Niranjan Y. Sardesai
26. Electroporation-Mediated Administration of Candidate DNA Vaccines against HIV-1
Sandhya Vasan
27. Electroporation-Mediated Intradermal Delivery of DNA Vaccines in Nonhuman Primates
Lucille Adam, Roger Le Grand, and Frederic Martinon
28. Intramuscular DNA Vaccination Protocols Mediated by Electric Fields Pieranna
Pieranna Chiarella and Emanuela Signori
29. DNA Electroporation of Multi-Agent Vaccines Conferring Protection Against Select Agent Challenge-TriGrid Delivery System
Andrea Keane-Myers, Matt Bell, Drew Hannaman, and Mark Albrecht
30. Gene-Based Vaccination and Screening Methods to Develop Monoclonal Antibodies
Pirouz Daftarian, Aram Vosoughi, and Vance Lemmon
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: February, 2014
Pages: 355
Weight: 8269g
Availability: Available
Subcategories: Genetics
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