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Main description:
Hands-on experts describe in step-by-step detail the key methodologies of contemporary peptide synthesis and illustrate their numerous applications. The techniques presented include protocols for chemical ligation, the synthesis of cyclic and phosphotyrosine-containing peptides, lipoamino acid- and sugar-conjugated peptides, and peptide purification and analyses. Additional chapters detail methodologies and instrumentation for high-throughput peptide synthesis, many different applications of peptides as novel research tools and biological probes, and the design and application of fluorescent substrate-based peptides that can be used to determine the selectivity and activity of peptidases. A practical guide to the identification of proteins using mass spectrometric analyses of peptide mixtures is also included.
Contents:
Part I: Common Strategies
Fundamentals of Modern Peptide Synthesis
Muriel Amblard, Jean-Alain Fehrentz, Jean Martinez, and Gilles Subra
Chimerism: A Strategy to Expand the Utility and Applications of Peptides
John Howl
Part II: Synthetic Methodologies and Applications
Modification of Peptides and Other Drugs Using Lipoamino Acids and Sugars
Joanne T. Blanchfield and Istvan Toth
Synthesis of Linear, Branched, and Cyclic Peptide Chimera
Gabor Mezoe and Ferenc Hudecz
Synthesis of Cell-Penetrating Peptides for Cargo Delivery
Margus Pooga and UElo Langel
Incorporation of the Phosphotyrosyl Mimetic 4(Phosphonodifluoromethyl)phenylalanine (F2Pmp) Into Signal Transduction-Directed Peptides
Zhu-Jun Yao, Kyeong Lee, and Terrence R. Burke, Jr.
Expressed Protein Ligation for Protein Semisynthesis and Engineering
Zuzana Machova and Annette G. Beck-Sickinger
Cellular Delivery of Peptide Nucleic Acid by Cell-Penetrating Peptides
Kalle Kilk and UElo Langel
Quenched Fluorescent Substrate-Based Peptidase Assays
Rebecca A. Lew, Nathalie Tochon-Danguy, Catherine A. Hamilton, Karen M. Stewart, Marie-Isabel Aguilar, and A. Ian Smith
A Convenient Method for the Synthesis of Cyclic Peptide Libraries
Gregory T. Bourne, Jonathon L. Nielson, Justin F. Coughlan, Paul Darwen, Marc R. Campitelli, Douglas A. Horton, Andreas Rhumann, Stephen G. Love, Tran T. Tran, and Mark L. Smythe
High-Throughput Peptide Synthesis
Michal Lebl and John Hachmann
Backbone Amide Linker Strategies for the Solid-Phase Synthesis of C-Terminal Modified Peptides
Jordi Alsina, Steven A. Kates, George Barany, and Fernando Albericio
Synthesis of Peptide Bioconjugates
Ferenc Hudecz
Part III: Practical Guides
Protein Identification by Mass Spectrometric Analyses of Peptides
AshleyMartin
Manual Solid-Phase Synthesis of Glutathione Analogs: A Laboratory-Based Short Course
Ursel Soomets, Mihkel Zilmer, and UElo Langel
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 274
Weight: 407g
Availability: Available
Subcategories: Biochemistry
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