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Main description:
Innate DNA and RNA Recognition: Method and Protocols presents validated experimental strategies to dissect nucleic acid sensing in-vitro and in-vivo sources. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Innate DNA and RNA Recognition: Method and Protocols provides a resource for immunologists, molecular biologists, virologists, microbiologists and researchers studying how the innate immune system handles nucleic acids from endogenous or foreign sources.
Feature:
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Back cover:
Innate DNA and RNA Recognition: Method and Protocols presents validated experimental strategies to dissect nucleic acid sensing in-vitro and in-vivo sources. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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Authoritative and practical, Innate DNA and RNA Recognition: Method and Protocols provides a resource for immunologists, molecular biologists, virologists, microbiologists, and researchers studying how the innate immune system handles nucleic acids from endogenous or foreign sources.
Contents:
Part I: Analysis of Viral Nucleic Acid Sensing In-Silico and In-Vitro
1. Detection of RNA Modifications by HPLC Analysis and Competitive ELISA
Gernot Nees, Andreas Kaufmann and Stefan Bauer
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2. Enzymatic Synthesis and Purification of a Defined RIG-I Ligand
Marion Goldeck, Martin Schlee, Gunther Hartmann, and Veit Hornung
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3. Crystallization of Mouse RIG-I ATPase Domain: In situ Proteolysis
Filiz Civril and Karl-Peter Hopfner
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4. Isolation of RIG-I-associated RNAs from Virus Infected Cells
Andreas Schmidt, Andreas Linder, Nicolas Linder, and Simon Rothenfußer
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5. Structure Modeling of Toll-like Receptors
Jing Gong and Tiandi Wei
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6. Nucleic Acid Recognition in Dendritic Cells
Alexander Heiseke, Katharina Eisenächer, and Anne Krug
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7. Viral Nucleic Acid Recognition in Human Non-immune cells: In vitro Systems
Andrea Ribeiro and Markus Wörnle
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8. Analysis of Nucleic Acid-induced Non-immune Cell Death in Vitro
Simone Romoli, Adriana Migliorini
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9. In vitro Analysis of Nucleic Acid Recognition in B Lymphocytes
Saskia Ziegler and Isabelle Bekeredjian-Ding
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10. Mapping of Optimal CD8 T Cell Epitopes
Julia Roider, Thomas Vollbrecht and Rika Draenert
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11. A Modular Approach to Suppression Ass says: TLR-Ligands, Conditioned Medium, and Viral Infection
Viktor H. Koelzer and David Anz
 12. MicroRNA Methodology: Advances in miRNA-technologiesTheresa Kaeuferle, Sabine Bartel, Stefan Dehmel, and Susanne Krauss-Etschmann
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Part II: Analysis of Nucleic Acid Sensing In-Vivo
13. Expression Profiling by Real-time Quantitative Polymerase Chain Reaction (RT-qPCR)
Maciej Lech and Hans-Joachim Anders
 14. Evaluating the Role of Nucleic Acid Antigens in Murine Models of Systemic Lupus ErythematosusAmanda A. Watkins, Ramon G. B. Bonegio and Ian R. Rifkin
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15. Induction and Analysis of Nephrotoxic Serum Nephritis in Mice
John M. Hoppe and Volker Vielhauer
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16. Isolation of Intratumoral Leukocytes of TLR-stimulated Tumor-bearing Mice
Moritz Rapp, David Anz, and Max Schnurr
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17. Bifunctional siRNAs for Tumor Therapy
Fanny Matheis and Robert Besch
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PRODUCT DETAILS
Publisher: Springer (Springer New York)
Publication date: June, 2014
Pages: 170
Weight: 601g
Availability: Not available (reason unspecified)
Subcategories: Genetics
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