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Main description:
The rapid pace of microRNA (miRNA) research continues to drive the advances of techniques for miRNA expression profiling, and innovative technologies that are more sensitive, specific, quantitative, and that are compatible with a wide range of biospecimens have been developed during the past few years. In Next-Generation MicroRNA Expression Profiling Technology: Methods and Protocols, expert researchers in the field contribute detailed examinations of the most current approaches being used today. This volume includes comprehensive coverage of methodologies that have been developed for miRNA profiling, as well as next-gen sequencing technology, miRNA databases, and specialized applications, such as cancer studies and miRNA-based non-invasive biomarker development. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Next-Generation MicroRNA Expression Profiling Technology: Methods and Protocols provides an ideal guide for novices and experts alike who are seeking to utilize these powerful technologies.
Contents:
1. Controlling miRNA Regulation in Disease
Willemijn M. Gommans and Eugene Berezikov
2. Introduction to miRNA Profiling Technologies and Cross-Platform Comparison
Sarah Aldridge and James Hadfield
3. Stem-Loop RT-qPCR for MicroRNA Expression Profiling
James Hurley, Doug Roberts, Andrew Bond, David Keys, and Caifu Chen
4. Poly(T) Adaptor RT-PCR
Rui Shi, Ying-Husan Sun, Xing-Hai Zhang, and Vincent L. Chiang
5. MicroRNA In Situ Hybridization
Boye Schnack Nielsen
6. Agilent MicroRNA Microarray Profiling System
Petula N. D'Andrade and Stephanie Fulmer-Smentek
7. miRNA Gene Expression Profiling Using Illumina Universal BeadChips
Jing Chen, Craig S. April, and Jian-Bing Fan
8. MicroRNA Expression Analysis Using the Affymetrix Platform
Suzanne Dee and Robert C. Getts
9. Individualized miRNA Assay Panels Using Optically Encoded Beads
Keld Sorensen
10. Microfluidic Primer Extension Assay (MPEA)
Markus Beier and Valesca Boisguerin
11. MicroRNA Profiling Using Paraflo Microfluidic Array Technology
Xiaochuan Zhou, Qi Zhu, Christoph Eicken, Nijing Sheng, Xiaolin Zhang, Litao Yang, and Xiaolian Gao
12. MicroRNA Expression Analysis Using the Illumina MicroRNA-Seq Platform
Shujun Luo
13. Next Generation Sequencing of miRNAs with Roche 454 GS-FLX Technology: Steps for a Successful Application
Ana Raquel Soares, Patricia M. Pereira, and Manuel A.S. Santos
14. Methods for Small RNA Preparation for Digital Gene Expression Profiling by Next-Generation Sequencing
Sam E.V. Linsen and Edwin Cuppen
15. Profiling of Short RNAs Using Helicos Single-Molecule Sequencing
Philipp Kapranov, Fatih Ozsolak, and Patrice M. Milos
16. deepBase: Annotation and Discovery of microRNAs and Other Non-Coding RNAs from Deep Sequencing Data
Jian-Hua Yang and Liang-Hu Qu
17. PhenomiR: MicroRNAs in Human Diseases and Biological Processes
Andreas Ruepp, Andreas Kowarsch, and Fabian Theis
18. miRNA Expression Profiling: From Reference Genes to Global Mean Normalization
Barbara D'haene, Pieter Mestdagh, Jan Hellemans, and Jo Vandesompele
19. miRNA Data Analysis: Next-Gen Sequencing
Preethi H. Gunaratne, Cristian Coarfa, Benjamin Soibam, and Arpit Tandon
20. Integrated miRNA Expression Analysis and Target Prediction
William Ritchie and John E.J. Rasko
21. miRNAs in Human Cancer
Xiaomin Zhong, George Coukos, and Lin Zhang
22. Blood-Based miRNA Preparation for Non-Invasive Biomarker Development
Svenja Debey-Pascher, Jing Chen, Thorsten Voss, and Andrea Staratschek-Jox
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: December, 2011
Pages: 352
Weight: 867g
Availability: Available
Subcategories: Genetics
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