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Main description:
This volume reviews available mouse models of atherosclerosis, basic aspects of disease initiation and progression, and the visualization of atherosclerotic lesions with invasive and non-invasive techniques that are widely used both in basic research and in the clinic. The chapters catalogue a wide range of in vitro and in vivo experimental methods used for atherosclerosis research, including thorough protocols and valuable notes based on the authors' personal wet lab experience. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Methods in Mouse Atherosclerosis will be a valuable working guide for researchers performing mouse-based atherosclerosis studies.
Contents:
1. Use of Mouse Models in Atherosclerosis Research
Godfrey S. Getz and Catherine A. Reardon
2. Cytokines and Immune Responses in Murine Atherosclerosis
P.J.H. Kusters and E. Lutgens
3. Intravital Microscopy for Atherosclerosis Research
Remco T. A. Megens and Oliver Soehnlein
4. Non-invasive Molecular Imaging of Mouse Atherosclerosis
Martina A. McAteer and Robin P. Choudhury
5. Oil Red O and Hematoxylin and Eosin Staining for Quantification of Atherosclerosis Burden in Mouse Aorta and Aortic Root
M. Jesus Andres-Manzano, Vicente Andres, and Beatriz Dorado
6. Isolation, Culture, and Polarization of Murine Bone Marrow-Derived and Peritoneal Macrophages
Ines Pineda-Torra, Matthew Gage, Alba de Juan, and Oscar M. Pello
7. Isolation of Mouse Primary Aortic Endothelial Cells by Selection with Specific Antibodies
Pedro Molina-Sanchez and Vicente Andres
8. Isolation and Culture of Aortic Smooth Muscle Cells and In Vitro Calcification Assay
Ricardo Villa-Bellosta and Magda R. Hamczyk
9. Immunostaining of Macrophages, Endothelial Cells, and Smooth Muscle Cells in the Atherosclerotic Mouse Aorta
Prashanthi Menon and Edward A. Fisher
10. Immunostaining of Lymphocytes In Mouse Atherosclerotic PlaqueAnton Gistera and Daniel F.J. Ketelhuth
11. Flow Cytometric Analysis of Immune Cells Within Murine Aorta
Breanne N. Gjurich, Pare sa L. Taghavie-Moghadam, and Elena V. Galkina
12. In Vitro Differentiation of Naive CD4+ T cells: A Tool for Understanding the Development of Atherosclerosis
Salvador Iborra and Jose M. Gonzalez-Granado
13. Quantification of Apoptosis in Mouse Atherosclerotic Lesions
Nichola L Figg and Martin R Bennett
14. Quantification of Cellular Proliferation in Mouse Atherosclerotic Lesions
Jose J. Fuster
15. Quantification of in vitro Macrophage Cholesterol Efflux and in vivo Macrophage-Specific Reverse Cholesterol Transport
Joan Carles Escola-Gil, Miriam Lee-Rueckert, David Santos, Lidia Cedo, Francisco Blanco-Vaca , and Josep Julve
16. In vitro Macrophage Phagocytosis Assay
Magda R. Hamczyk, Ricardo Villa-Bellosta, Vicente Andres
17. Glucose and Insulin Tolerance Tests in the Mouse
Angela Vinue and Herminia Gonzalez-Navarro
18. Wire Myography to Study Vascular Tone and Vascular Structure of Isolated Mouse Arteries
Lara del Campo and Mercedes Ferrer
19. Pressure Myography to Study the Function and Structure of Isolated Small Arteries
Olav L. Schjorring, Rune Carlsson, and Ulf Simonsen
20. Generation of Aorta Transcript Atlases of Wild-type and Apolipoprotein E-null Mice by Laser Capture Microdissection-based mRNA Expression Microarrays
Changjun Yin, Sarajo Mohanta , Zhe Ma, Christian Weber, Desheng Hu, Falk Weih, and Andreas Habenicht
21. Analysis of Gene and Protein Expression In Atherosclerotic Mouse Aorta By Western Blot and Quantitative Real-Time PCR
Jose Rivera-Torres
22. Bone Marrow Transplantation in Mice to Study the Role of Hematopoietic Cells in Atherosclerosis
Vinatha Sreeramkumar and Andres Hidalgo
23. Tandem Stenosis to Induce Atherosclerotic Plaque Instability in the Mouse
Chen Y.C., Rivera J., and Peter K.
24. Detection of Intraplaque Hemorrhage in Mouse Atherosclerotic Lesions
Judith C. Sluimer, Marion J. Gijbels, and Sylvia Heeneman
25. Mechanical Stabilization of Mouse Carotid Artery for in vivo intravital Microscopy Imaging of Atherogenesis
Raphael Chevre
26. Intravital Microscopy in the Cremaster Muscle Microcirculation for Endothelial Dysfunction Studies
Cristina Rius and Maria J. Sanz
27. Fluorescent Molecular Tomography for in vivo Imaging of Mouse Atherosclerosis
Alicia Arranz, Markus Rudin, Carlos Zaragoza, and Jorge Ripoll
28. In vivo 18F-FDG-PET Imaging in Mouse Atherosclerosis
Jesus Mateo, Izaskun Bilbao, Juan Jose Vaquero, Jesus Ruiz-Cabello, and Samuel Espana
29. Magnetic Resonance Imaging of the Atherosclerotic Mouse Aorta
Jesus Mateo, Marina Benito, Samuel Espana, Javier Sanz, Jesus Jimenez-Borreguero Valentin Fuster, and Jesus Ruiz-Cabello
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: October, 2015
Pages: 355
Weight: 652g
Availability: Available
Subcategories: Cardiovascular Medicine, General Issues
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