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Main description:
Understanding the molecular and cellular mechanisms underlying the development of specific neural circuits is not just an intellectual curiosity but also central to our ability to develop therapeutic approaches to repair damaged pathways in the future. In Neural Development: Methods and Protocols, experts in the field contribute commonly used protocols to facilitate future research in developmental neuroscience. Split into four convenient sections, this detailed volume covers techniques of culturing neurons and glia as well as their growth and differentiation, methods of gene delivery and down regulation, protocols for analyzing axon growth and guidance plus synapse formation, and, finally, basic methods to analyze brain morphology and axon pathways in developing animals. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and accessible, Neural Development: Methods and Protocols provides key guidance for students and postdoctoral fellows new to developmental neurobiology.
Contents:
Part I: Neuron and Glial Cell Culture
1. Isolation and Maintenance of Cortical Neural Progenitor Cells In Vitro
Mi-Yeon Kim, Byoung-San Moon, and Kang-Yell Choi
2. Neural Induction with a Dopaminergic Phenotype from Human Pluripotent Stem Cells Through a Feeder-Free Floating Aggregation Culture
Asuka Morizane, Daisuke Doi, and Jun Takahashi
3. Nucleoside Analog Labeling of Neural Stem Cells and Their Progeny
Erik DeBoer and Mladen-Roko Rasin
4. Culture of Dissociated Hippocampal Neurons
Yun Peng, Wen-Cheng Xiong, and Lin Mei
5. Culturing Mouse Cerebellar Granule Neurons
Tharakeswari Selvakumar and Daniel L. Kilpatrick
6. Preparation of Primary Cultured Dopaminergic Neurons from Mouse Brain
Won-Seok Choi, Hyung-Wook Kim, and Zhengui Xia
7. Culturing Astoryctes from Postnatal Rats
Yangyang Huang and Cheryl Dreyfus
8. Culturing Oligodendrocyte Lineage Cells from Neonatal Rats
Clifton G. Fulmer and Cheryl F. Dreyfus
9. Isolation and Culture of Schwann Cells
Yanmei Tao
Part II: Gene Expression and Analysis
10. DNA Transfection: Calcium Phosphate Method
Munjin Kwon and Bonnie L. Firestein
11. The Gene-Gun Approach for Transfection and Labeling of Cells in Brain Slices
Anna Dunaevsky
12. Lentiviral Vector Production, Titration, and Transduction of Primary Neurons
Baojin Ding and Daniel L. Kilpatrick
13. In-Ovo Electroporation in Embryonic Chick Spinal Cords
Hui Wang and Michael P. Matise
14. Gene Transfer in Developing Chick Embryos: In Ovo Electroporation
Emma K. Farley
15. In Utero Electroporation in Mice
Chunlei Wang and Lin Mei
16. General Introduction to In Situ Hybridization Protocol Using Non-Radioactively Labeled Probes to Detect mRNAs on Tissue Sections
Daehoon Lee, Shan Xiong, and Wen-Cheng Xiong
17. Immunofluorescence Staining with Frozen Mouse or Chick Embryonic Tissue Sections
Hui Wang and Michael P. Matise
18. -Galactosidase Staining of LacZ Fusion Proteins in Whole Tissue Preparations
Margaret A. Cooper and Renping Zhou
19. Chromatin Immunoprecipitation Assay of Brain Tissues Using Percoll Gradient-Purified Nuclei
Baojin Ding and Daniel L. Kilpatrick
20. In Vivo Dual Luciferase Reporter Assay with Chick Neural Tube In Ovo Electroporation System
Hui Wang and Michael P. Matise
Part III: Axon Pathways and Synapses
21. Growth Cone Collapse Assay
Xin Yue, Alexander I. Son, and Renping Zhou
22. The Stripe Assay: Studying Growth Preference and Axon Guidance on Binary Choice Substrates In Vitro
Markus Weschenfelder, Franco Weth, Bernd Knoell, and Martin Bastmeyer
23. Microcontact Printing of Substrate-Bound Protein Patterns for Cell- and Tissue Culture
Martin Fritz and Martin Bastmeyer
24. Semi-Automated Analysis of Dendrite Morphology in Cell Culture
Eric S. Sweet, Chris L. Langhammer, Melinda K. Kutzing, and Bonnie L. Firestein
25. Monitoring Synaptic Plasticity by Imaging AMPA Receptor Content and Dynamics on Dendritic Spines
Hiroshi Makino and Bo Li
26. Morphological Analysis of Neuromuscular Junctions by Immunofluorescent Staining of Whole-Mount Mouse Diaphragms
Haitao Wu and Lin Mei
Part IV: Morphological Analyses of the Developing Nervous System
27. Routine Histology Techniques for the Developing and Adult Central Nervous System
W. Geoffrey McAuliffe
28. Cryosectioning
Alexander I. Son, Katie Sokolowski, and Renping Zhou
29. The Golgi-Cox Method
Gitanjali Das, Kenneth Reuhl, and Renping Zhou
30. Neuroanatomical Tract-Tracing Using Biotinylated Dextran Amine
Nikolai E. Lazarov
31. Retrograde Tracing Technique for Neonatal Animals
Kengo Funakoshi, Akira Yoshikawa, and Yoshitoshi Atobe
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: May, 2013
Pages: 322
Weight: 8269g
Availability: Available
Subcategories: Neuroscience
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