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Main description:
The in situ hybridization and PCR technologies are now well-established molecular techniques for studying chromosomal aneuploidy and rearran- ments, gene localization and expression, and genomic organization. Over the last decade, we have seen increasing applications in these fields. By combining the high sensitivity of the PCR reaction and the cytological localization of target sequences, both PRINS and in situ PCR techniques have provided highly powerful complements to FISH for in situ cellular and molecular investigations. Both these approaches have several advantages in terms of sensitivity and specificity, owing to the use of primers and to the fast kinetics of annealing and elongation reactions in situ. In the first edition of PRINS and In Situ PCR Protocols edited by John R. Gosden, experts in the field presented in detail a variety of applications of PRINS and in situ PCR techniques, in a wide range of clinical conditions. Since the publication of this successful reference book, there have been s- nificant improvements in in situ detection techniques. This completely revised and updated second edition presents a compreh- sive selection of new procedures developed in the field of PRINS and in situ PCR technologies. The book has two sections. Part I, Basic Methodology, contains chapters that provide useful protocols for many variations of PRINS and in situ PCR, including a new fast multicolor PRINS method, and protocols for PRINS detection of unique sequences in situ.
Contents:
Part I. Basic Methodology
New Rapid Multicolor PRINS Protocol
Ju Yan, Macoura Gadji, Kada Krabchi, and Regen Drouin
PRINS Combined With Peptide Nucleic Acid Labeling
Franck Pellestor and Petra Paulasova
PRINS Combined With Indirect Immunofluorescence
Philippe Coullin
PRINS for the Detection of Unique Sequences
Stephen S. Wachtel and Avirachan T. Tharapel
Single-Copy and Point Mutation Analysis (Rolling-Circle PRINS)
Jorn Koch
Analysis of Sperm Aneuploidy by PRINS
Franck Pellestor, Brigitte Andreo, Jacques Puechberty, Genevieve Lefort, and Pierre Sarda
In Situ PCR Protocols
Omar Bagasra and Twaina Harris
Part II. Research and Clinical Applications
Detection and Sizing of Telomeric and Other Simple Repeats by Dideoxy-PRINS
Jorn Koch
PRINS Evaluation of Chromosome Instability in Mammalian Cells by Detection of Repetitive DNA Sequences in Micronuclei
Antonella Russo
PRINS for the Detection of Gene Deletions in Cancer
Avirachan T. Tharapel and Stephen S. Wachtel
Combined Fluorescence In Situ Hybridization and PRINS for the Analysis of the Dystrophin Gene
Liborio Stuppia, Dario La Sala, and Caterina Cinti
Brain Tissue Preparations for Chromosomal PRINS Labeling
Ivan Y. Iourov, Svetlana G. Vorsanova, Franck Pellestor, and Yuri B. Yurov
PRINS on Plant Chromosomes
Jiri Macas, Alice Navratilova, Marie Kubalakova, and Jaroslav Dolezel
Dual-Color PRINS for In Situ Detection of Fetal Cells in Maternal Blood
Kada Krabchi, Macoura Gadji, Ju Yan, and Regen Drouin
PRINS as an Efficient Tool for Aneuploidy Assessment in Human Oocytes and Preimplantation Embryos
Franck Pellestor, Brigitte Andreo, Tal Anahory, and Samir Hamamah
Microdissection With PCR In Situ
Allen T. Christian and ChristineHara
Application of Reverse Transcription In Situ PCR in Cancer Analysis
Tamotsu Takeuchi, Yoshihiro Adachi, Hiroshi Sonobe, and Yuji Ohtsuki
In Situ Reverse Transcription PCR on Plant Tissues
Zbigniew Przybecki, Ewa Siedlecka, Marcin Filipecki, and Ewa Urbanczyk-Wochniak
In Situ PCR for the Detection of Human Cytomegalovirus in Suspension Cells During the Latent Phase of Infection
Barry Slobedman
Development and Adaptation of the PRINS Technology: An Overview
Franck Pellestor
Latest Developments in In Situ PCR
Omar Bagasra and Twaina Harris
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: December, 2010
Pages: 244
Weight: 447g
Availability: Available
Subcategories: Biochemistry
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