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Main description:
Over the past decades, knowledge about the cellular and molecular basis underlying the visual process has remarkably increased. In Retinal Degeneration: Methods and Protocols, expert researchers in the field provide a guide of relevant and state-of-the-art methods for studying retinal homeostasis and disease. These include methods and techniques for addressing cell culture systems and animal models of disease, their generation, their phenotypic and molecular characterization as well as their use in therapeutic approaches to the retina. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Retinal Degeneration: Methods and Protocols aids scientists in continuing to study the cutting-edge techniques of retinal cell biology in health and disease.
Contents:
Part I: Human Genetics
1. Identification and Analysis of Inherited Retinal Disease Genes
Kornelia Neveling, Anneke I. den Hollander, Frans P.M. Cremers, and Rob W.J. Collin
Part II : Mouse Models
2. Mouse Models for Studies of Retinal Degeneration and Diseases
Bo Chang
3. Retinal Fundus Imaging in Mouse Models of Retinal Diseases
Anne F. Alex, Peter Heiduschka. Nicole Eter
4. Functional Phenotyping of Mouse Models with ERG
Naoyuki Tanimoto, Vithiyanjali Sothilingam, and Mathias W. Seeliger
5. Phenotyping of Mouse Models with OCT
M. Dominik Fischer, Ahmad Zhour and Christoph J. Kernstock
6. Light Damage as a Model of Retinal Degeneration
Christian Grimm and Charlotte E. Reme
7. N-Methyl-D-Aspartate (NMDA)-mediated Excitotoxic Damage - A Mouse Model of Acute Retinal Ganglion Cell Damage
Roswitha Seitz and Ernst R. Tamm
Part III: Other Animal Models
8. Generation of Transgenic X. laevis Models of Retinal Degeneration
Beatrice M. Tam, Christine C-L. Lai, Zusheng Zong and Orson L. Moritz
9. Analysis of Photoreceptor Degeneration in the Zebrafish Danio rerio
Holger Dill, Bastian Linder, Anja Hirmer and Utz Fischer
10. Analysis of Optokinetic Response in Zebrafish by Computer Based Eye Tracking
Sabina P. Huber-Reggi, Kaspar P. Mueller, Stephan C.F. Neuhauss
11. Analysis of the Drosophila Compound Eye with Light and Electron Microscopy
Monalisa Mishra and Elisabeth Knust
Part IV : In Situ Analyses
12. Cell Specific Markers for the Identification of Retinal Cells by Immunofluorescence Microscopy
Christiana L. Cheng, Hidayat Djajadi and Robert S. Molday
13. A Method of Horizontally-Sliced Preparation of the Retina
Ryosuke Enoki and Amane Koizumi
14. Detection of DNA Fragmentation in Retinal Apoptosis by TUNEL
Francesca Doonan and Thomas G. Cotter
15. High-throughput RNA in situ Hybridization in Mouse Retina
Seth Blackshaw
16. Assessment of Mitochondrial Damage in Retinal Cells and Tissues using Quantitative Polymerase Chain Reaction for Mitochondrial DNA Damage and Extracellular Flux Assay for Mitochondrial Respiration Activity
Stuart G. Jarrett, Barbel Rohrer, Nathan R Perron, Craig Beeson, and Michael E. Boulton
17. Analysis of Photoreceptor Rod Outer Segment Phagocytosis by RPE Cells in situ
Saumil Sethna and Silvia C. Finnemann
Part V: Tissue Culture and Cell Models
18. Ca2+ Microfluorimetry in Retinal Muller Glial Cells
Antje Wurm, Thomas Pannicke, Andreas Reichenbach
19. Functional Analysis of Retinal Microglia and their Effects on Progenitors
Debra A Carter, Balini Balasubramaniam and Andrew D Dick
20. Analysis of Photoreceptor Outer Segment Phagocytosis by RPE Cells in Culture
Yingyu Mao and Silvia C. Finnemann
21. Ca2+ Imaging Techniques to Analyze Ca2+ Signaling in Cells and to Monitor Neuronal Activity in the Retina
Olaf Strauss
Part VI: Gene Regulation
22. Double Chromatin Immunoprecipitation: Analysis of Target Co-Occupancy of Retinal Transcription Factors
Guang-Hua Peng and Shiming Chen
23. Quantifying the Activity of cis-regulatory Elements in the Mouse Retina by Explant Electroporation
Cynthia L. Montana, Connie A. Myers, and Joseph C. Corbo
Part VII: Therapy
24. Optimized Technique for Subretinal Injections in Mice
Regine Muhlfriedel, Stylianos Michalakis, Marina Garcia Garrido, Martin Biel, Mathias. W. Seeliger
25. Adeno Associated Viral Vectors for Gene Therapy of Inherited Retinal Degenerations
John G. Flannery and Meike Visel
26. Barrier Modulation in Drug Delivery to the Retina
Matthew Campbell, Marian Humphries and Peter Humphries
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2012
Pages: 398
Weight: 8928g
Availability: Available
Subcategories: General Issues, Ophthalmology and Optometry
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