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MORE ABOUT THIS BOOK
Main description:
Lipases are the most applied enzymes in organic synthesis due to their broad substrate acceptance and because of the availability of the molecular, biochemical, themodynamical and solvent engineering tools, which allows the optimization of lipases and lipase-catalyzed reactions. On the other hand, phospholipases are emerging as useful enzymes in food and pharmaceutical industries. In Lipases and Phospholipases: Methods and Protocols, expert researchers in the field provide key techniques to investigate these essential enzymes. Focusing on fundamental issues, current and new applications as well as practical step-by-step protocols, and the extensive applications of lipases and the potential application of phospholipases and its inhibitors. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.
Thorough and intuitive, Lipases and Phospholipases: Methods and Protocols aids scientists in continuing to study lipases, phopholipases and related enzymes.
Feature:
Details fundamental issues and new applications of lipases and phospholipases
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Back cover:
Lipases are the most applied enzymes in organic synthesis due to their broad substrate acceptance and because of the availability of the molecular, biochemical, themodynamical and solvent engineering tools, which allows the optimization of lipases and lipase-catalyzed reactions. On the other hand, phospholipases are emerging as useful enzymes in food and pharmaceutical industries. In Lipases and Phospholipases: Methods and Protocols, expert researchers in the field provide key techniques to investigate these essential enzymes. Focusing on fundamental issues, current and new applications as well as practical step-by-step protocols, and the extensive applications of lipases and the potential application of phospholipases and its inhibitors. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.
Thorough and intuitive, Lipases and Phospholipases: Methods and Protocols aids scientists in continuing to study lipases, phopholipases and related enzymes.
Contents:
Part I: Overview of Lipases, Phospholipases and Related Enzymes
1. Lipases: An Overview Leticia Casas Godoy, Sophie Duquesne, Florence Bordes, Georgina Sandoval and Alain Marty
2. Lipases or Esterases: Does it Really Matter? Toward a New Bio-Physico-Chemical Classification Yassine Ben Ali, Robert Verger, and Abdelkarim Abousalham
3. Study of New Feruloyl Esterases to Understand Lipase Evolution: The Case of Bacillus flexus Mónica Sánchez-González, Allan Blanco-Gámez, Roberto Parra-Saldívar, Juan Carlos Mateos-Díaz and Maria Isabel Estrada Alvarado
4. Phospholipases: An Overview Ahmed Aloulou, Yassine Ben Ali, Sofiane Bezzine, Youssef Gargouri and Michael H. Gelb
Part II: Screening and Sources of Lipases, Phospholipases and Its Inhibitors
5. High-Throughput Sceening Method for Lipases/Esterases Eduardo Mateos-Díaz, Jorge Alberto Rodríguez, María de los Ángeles Camacho-Ruiz, Juan Carlos Mateos-Díaz
6. Functional-Based Screening Methods for Lipases, Esterases and Phospholipases in Metagenomic Libraries
Dolores Reyes-Duarte, Manuel Ferrer, and Humberto García-Arellano
7. Plant Lipases:Partial Purification of C. papaya Lipase
Ivanna Rivera, Juan Carlos Mateos-Díaz, and Georgina Sandoval
8. Plant Phospholipases: an Overview
Geliang Wang, Stephen Ryu, and Xuemin Wang
9. Lipase and Phospholipase Inhibitors: Design and Applications
Mark E. Venable
10. High-Throughput Assay of Secreted Phospholipases A2 Inhibitors
Wallace Thompson, Rob C. Oslund, James Bollinger, Heather Ewing and Michael H. Gelb
Part III Cloning, Production, Purification and Characterization of Lipases and Phospholipases
11. Heterologous Expression Systems for Lipases: A Review
Francisco Valero
12. Streptomyces Phospholipase D: Cloning and Production
Yozo Nakazawa
13. Cloning of PLD2 from Baculovirus for Studies in Inflammatory Responses
Julian Gomez-Cambronero and Karen M. Henkels
14. Lipases Production by Solid-State Fermentation: The Case of Rhizopus homothallicus in Perlite
Susana Velasco-Lozano, Tania Volke-Sepulveda, Ernesto Favela-Torres
15. Lipases and Esterases from Extremophiles: Overview and Case Example of the Production and Purification of an Esterase from Thermus thermophilus HB27
Pablo Fuciños, Ramón González, Estrella Atanes, Ana Belem Fernández Sestelo, Nelson Pérez-Guerra, Lorenzo Pastrana and María Luisa Rúa
16. Purification, Biochemical and Kinetic Properties of Recombinant
Staphylococcus aureus Lipase
Habib Horchani, Ahmed Fendri, Hanen Louatia, Adel Sayari, Youssef Gargouri and Robert Verger
17. Phospholipase A2 Purification and Characterization; A Case Study
Aida Karray, Youssef Gargouri, Robert Verger and Sofiane Bezzine
Part IV: Optimization of the Biocatalyst and Reaction Conditions
18. The Yeast Yarrowia lipolytica as a Generic Tool for Molecular Evolution of Lipases
Sophie Duquesne, Florence Bordes, Franck Fudalej, Jean-Marc Nicaud and Alain Martya
19. Molecular Modeling of Lipase Binding to a Substrate-Water Interface
Christian C. Gruber and Jürgen Pleiss
20. Different Strategies for Hyperactivation of Lipase Biocatalysts
Jose M. Palomo and Jose M. Guisan
21. Rational Design of Immobilized Lipases and Phospholipases
Alberto del Monte Martínez and Bessy V. Cutiño Avila
22. Thermodynamical Methods for the Optimization of Lipase Catalyzed Reactions
Edmundo Castillo, Alejandro Torres-Gavilán, Georgina Sandoval , Alain Marty
Part V: Applications of Lipases and Phopholipases
23. Lipases as Biocatalysts for the Synthesis of Structured Lipids Ram Chandra Reddy Jala, Peng Hu, Tiankui Yang, Yuanrong Jiang, Yan Zheng, Xuebing Xu
24. Lipases-Catalyzed Modification of Phenolic Antioxidants Pamela Torres, Dolores Reyes-Duarte, Antonio Ballesteros and Francisco J. Plou
25. Lipases as Catalysts in Synthesis of Fine Chemicals Alicia Baldessari
26. Lipases in Green Chemistry: Acylation and Alcoholysis on Steroids and Nucleosides Alicia Baldessari and Luis E. Iglesias
27. Lipases as Biocatalyst for Biodiesel Production Xiaohu Fan, Xochitl Niheus, Georgina Sandoval
28. Biopolymer Synthesis Catalyzed by Lipases Karla A. Barrera-Rivera, Arturo Flores-Carreón, Antonio Martínez-Richa
29. Phospholipases in Food Industry-A Review Víctor Casado, Diana Martín, Carlos Torres, Guillermo Reglero
30. Lipase and Phospholipase Biosensors: A Review Enrique J. Herrera-López
PRODUCT DETAILS
Publisher: Springer (Humana Press)
Publication date: March, 2012
Pages: 551
Weight: 1243g
Availability: Not available (reason unspecified)
Subcategories: Biochemistry
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