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Main description:
Intracellular checkpoint controls constitute a network of signal transd- tion pathways that protect cells from external stresses and internal errors. Ext- nal stresses can be generated by the continuous assault of DNA-damaging agents, such as environmental mutagens, ultraviolet (UV) light, ionizing radiation, or the reactive oxygen species that can arise during normal cellular metabolism. In response to any of these assaults on the integrity of the genome, the activation of the network of checkpoint control pathways can lead to diverse cellular responses, such as cell cycle arrest, DNA repair, or elimination of the cell by cell death (apoptosis) if the damage cannot be repaired. Moreover, internal errors can occur during the highly orchestrated replication of the cellular genome and its distribution into daughter cells. Here, the temporal order of these cell cycle events must be strictly enforced-for example, to ensure that DNA replication is c- plete and occurs only once before cell division, or to monitor mitotic spindle assembly, and to prevent exit from mitosis until chromosome segregation has been completed. Thus, well functioning checkpoint mechanisms are central to the maintenance of genomic integrity and the basic viability of cells and, the- fore, are essential for proper development and survival. The importance of proper functioning of checkpoints becomes plainly obvious under conditions in which this control network malfunctions and fails. Depending on the severity and timing, failure of this machinery can lead to embryonic lethality, genetic diseases, and cancer.
Contents:
Part I. Reviews of Checkpoint Controls, Their Involvement in the Development of Cancer, and Approaches to Their Investigation
G1 and S-Phase Checkpoints, Chromosome Instability, and Cancer
Hiroshi Nojima
Analyzing the G2/M Checkpoint
George R. Stark and William R. Taylor
Analyzing the Spindle Checkpoint in Yeast and Frogs
P. Todd Stukenberg and Daniel J. Burke
Cell Cycle Checkpoint Control Mechanisms That Can Be Disrupted in Cancer
Bipin C. Dash and Wafik El-Deiry
Part II. Analyzing Checkpoint Controls in Diverse Model Systems
Establishment of a Cell-Free System to Study the Activation of Chk2
Xingzhi Xu and David F. Stern
Analyzing Checkpoint Controls in Human Skin
Sandra Pavey and Brian G. Gabrielli
Generation and Analysis of Brca1 Conditional Knockout Mice
Chu-Xia Deng and Xiaoling Xu
Analysis of Cell Cycle Progression and Genomic Integrity in Early Lethal Knockouts
Eric J. Brown
Xenopus Cell-Free Extracts to Study the DNA Damage Response
Vincenzo Costanzo, Kirsten Robertson, and Jean Gautier
A Xenopus Cell-Free System for Functional Analysis of the Chfr Ubiquitin Ligase Involved in Control of Mitotic Entry
Dongmin Kang, Jim Wong, and Guowei Fang
Control of Mitotic Entry After DNA Damage in Drosophila
Burnley Jaklevic, Amanda Purdy, and Tin Tin Su
Methods for Analyzing Checkpoint Responses in Caenorhabditis elegans
Anton Gartner, Amy J. MacQueen, and Anne M. Villeneuve
Assaying the Spindle Checkpoint in the Budding Yeast Saccharomyces cerevisiae
Christopher M. Yellman and Daniel J. Burke
Purification and Analysis of Checkpoint Protein Complexes From Saccharomyces cerevisiae
Catherine M. Green and Noel F. Lowndes
Index
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2010
Pages: 334
Weight: 572g
Availability: Available
Subcategories: Oncology
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